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May 8, 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-. creERT2 (43). Mice treated with tamoxifen and monitored for 4,. 5, or 7 d showed a

Mice treated with tamoxifen and monitored for 4,. 5, or 7 d showed a In sharp contrast to control SPA-Cre; Cdh1fl/+; Lgr5-eGFP-IRES-CreERT2 mice, where GFP- positive Lgr5+ cells resided at the base of the glands in both the Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mice. To label only Lgr5 stem cells, Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mouse was injected with. For genetic lineage tracing, Lgr5-EGFP-IRES-cre/ERT2 mice were crossed with Rosa26-tdTomato mice. To induce the expression of the reporter gene, a single We crossed Lgr5-EGFP-IRES-CreERT2 knock-in and ROSA26-tdTomato (LRT) mice, and treated them with or without 4-hydroxytamoxifen (4-OHT), to determine Aug 1, 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti Exogenous IFN-γ depletes Paneth cells and modifies the expression of rapidly cycling ISC markers.

Lgr5-egfp-ires-creert2

Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture. Adaptations to defined media components in the wake of advances in ES and iPS cell culture has led to the identification of conditions that maintained intestinal cell progenitors in culture. The mammalian intestinal epithelium is endowed with a high cell turnover sustained by a few stem cells located in the bottoms of millions of crypts. Until recently, it was generally assumed that the extreme sensitivity to DNA damaging agents leading to cell death and the asymmetric mode of chromosome segregation of intestinal epithelial stem cells prevented the illicit survival of mutated stem Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mice. To label only Lgr5 stem cells, Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mouse was injected with. 8 May 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-. creERT2 (43).

EGFP-ires-CreERT2 kidney sections using anti-GFP immunohis- tochemistry (IHC) (Figure To measure the overlap of Krt19+ cells with Lgr5+ cells, we performed Krt19 in situ hybridization on colonic (Figure 2A) tissues of Lgr5-EGFP-IRES-CreERT2 Methods Studies were performed in Lgr5−EGFP-IRES-creERT2 , Bmi1-CreERT2 , Hopx-CreERT2 , and TRE-H2BGFP::Hopx-CreERT2::lox-stop-lox-tdTomato determined the effects of the chemoprotective FPA diet on miRNAs and mRNAs in colonic stem cells obtained from Lgr5-EGFP-IRES-creERT2 knock-in mice.

2012-09-27 · Lgr5-EGFP-Ires-CreERT2, Lgr5-lacZ, and Rosa26-4color mice were generated as described previously (Barker et al., 2007). Rosa26RlacZ mice were obtained from Jackson Labs. All animal experiments were approved by the Animal Experimentation Committee of the Royal Dutch Academy of Science and the Institutional Animal Care and Use Committee of Singapore.

Expression of Bmi1, another putative N et al. Biol Res P 6 13 G007‑LKeatment repressed lineage tracing from˜ LGR5+ intestinal stem cells Lgr5-EGFP-Ires-CreERT2;R26R-Confettitdoublettrans- genictmicet adult Lgr5-EGFP-ires-CreERT2/Rosa four-color mice. Confocal.

By using the knock-in allele Lgr5EGFP-Ires-CreERT2, we have shown that the cell surface receptor Lgr5 (leucine-rich repeat–containing heterotrimeric guanine nucleotide–binding protein–coupled

We compared Lrig1 and Lgr5 expression, as Lgr5 is an established SC 2 Feb 2018 The regenerative response of Lgr5-positive ISC was examined by lineage tracing assay using Lgr5-EGFP-ires-CreERT2-TdT mice with Vav3−/− (Lgr5 APC V2V3) and Lgr5-EGFP-IRES-creERT2 Apcfl/fl. Tiam1. −/−. (Lrg5 APC T) mice, examining the role of these GEFs when Apc is deleted 12 Jun 2012 cells can be explored by use of the Lgr5-EGFP-ires-CreERT2 knock-in (Lgr5-ki) mouse (Supplementary Figure S1A; Barker et al, 2007). Lgr5-EGFP-IRES-. creERT2 mice were generated by homologous recombination in embryonic stem cells targeting an EGFP-IRES-creERT2 cassette to the ATG of We then crossed the stem-cell-specific Lgr5-EGFP-IRES-creERT2 knockin mice to Apcflox/flox mice. In the resulting mice, Cre was.

Synonyms: Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5 tm1 (cre/ESR1)Cle. Gene: Lgr5-eGFP-IRES-CreERT2 mice, where GFP- positive Lgr5+ cells resided at the base of the glands in both the corpus and the antrum, GFP-expressing Lgr5+ cells were hardly observed in -Cre; Cdh1fl/fl; SPA Lgr5-eGFP-IRES-CreERT2 mice (Figure 1C). These results suggest that E-cadherin is essential for the Lgr5-EGFP-ires-creERT2 mice,11 APCﬂ/ﬂ mice,25 Axin2-lacZ mice,26 and C57B/6 wild-type mice (6–12 weeks old) were used for experiments. Lgr5-EGFP-ires-creERT2 mice were crossed with APCﬂ/ﬂ mice. Cre enzyme activity was induced by intraperitoneal injections of tamoxifen (2 … 2007-11-01 Intestinal cell fractions from Lgr5-EGFP-ires-CreERT2 mice, expressing GFP under the control of the Lgr5 promoter.
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15).

creERT2 mice were generated by homologous recombination in embryonic stem cells targeting an EGFP-IRES-creERT2 cassette to the ATG of We then crossed the stem-cell-specific Lgr5-EGFP-IRES-creERT2 knockin mice to Apcflox/flox mice.
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Lgr5EGFP-Ires-CreERT2/Apc fl/flmice with the R26R-Confetti strain. Upon stochastic Cre induction by low-dose tamoxifen in adult mice, intestinal ade-nomas developed from Apc-deleted Lgr5+ stem cells.After4weeks,weobservedlargeadenomas (fig. S2, A to C) that either uniformly expressed a single Confetti colororconsistedofanatomically

2 a) in the majority (69.9%) of the villi (Additional file 3 : Figure S2A). 2007-10-14 · Figure 4: EGFP expression in an Lgr5-EGFP-IRES-creERT2 knock-in mouse faithfully reproduces the Lgr5-lacZ expression pattern in the intestinal tract. 实验动物商城平台. 在线客服 ; 实验动物一站式采购平台 cells in Lgr5-eGFP-IRES-CreERT2 mice.